Asian Journal of Transfusion Science
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Year : 2014  |  Volume : 8  |  Issue : 1  |  Page : 26-28

Sensitivity of individual and mini-pool nucleic acid testing assessed by dilution of hepatitis B nucleic acid testing yield samples

1 Department of Transfusion Medicine, All India Institute of Medical Sciences, New Delhi, India
2 Hemogenomics Pvt. Ltd, Bangalore, India

Correspondence Address:
Nitin Agarwal
Department of Transfusion Medicine, Blood Bank (Main), All India Institute of Medical Sciences, New Delhi
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Source of Support: Hemogenomics Pvt Ltd has helped financially to get the sample tested from a CAP accredited Lab., Conflict of Interest: Mr Sourit Chakroborty is an employee of Hemogenomics Pvt Ltd.

DOI: 10.4103/0973-6247.126684

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Introduction: For nucleic acid testing (NAT) of blood donations, either the blood samples can be pooled together in a batch of six or eight prior to testing (mini-pool-NAT [MP-NAT]), or the tests can be run on every individual sample (individual donor-NAT [ID-NAT]). It has been debated in various studies whether pooling of samples results in decreased sensitivity of detection as the volume of individual samples gets lesser in a pool. The objective of this study was to investigate the effect of dilution on the sensitivity of tests. Materials and Methods: The study was performed on nine plasma samples which were hepatitis B reactive exclusively by Procleix Ultrio Plus and not by Procleix Ultrio or serology. These nine exclusive UltrioPlus ID-NAT yield samples were diluted in 1:2, 1:4. 1:6 and 1:8 dilutions using previously tested negative plasma and each dilution of every sample along with archived undiluted sample were retested in three replicates with Procleix Ultrio Plus Assay. Results: Among NAT yield samples, 88.88% of the samples were detected when retested in ID-NAT in undiluted form. Samples with higher viral load (sample 5 and 6) were detected by all dilutions. When samples with viral load below 20 IU/mL were tested in dilutions of 1:6 or 1:8, only 9 out of 27 replicates (33.33%) were detected. This means that more than 67% of low viral load samples were missed by MP-NAT of 1:6 or 1:8 dilution out of total NAT yield samples. Conclusion: Individual Donor NAT is ideal methodology for NAT as dilution due to pooling may miss samples with low viral load as evident in this study.

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2006 - Asian Journal of Transfusion Science | Published by Wolters Kluwer - Medknow
Online since 10th November, 2006