Assessment of dengue virus inactivation in random donor platelets using amotosalen and ultraviolet A illumination Kumar A, Tiwari AK, Kumar S, Biswas A, Singh G, Chatterjee K, Chakroborty S, Sunil S,

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Assessment of dengue virus inactivation in random donor platelets using amotosalen and ultraviolet A illumination Ankit Kumar¹, Aseem Kumar Tiwari², Satendra Kumar³, Ashutosh Biswas⁴, Gurpreet Singh³, Kabita Chatterjee⁵, Sourit Chakroborty³, Sujatha Sunil¹ ¹ Vector Borne Diseases Group, International Centre For Genetic Engineering And Biotechnology, Aruna Asaf Ali Marg, New Delhi, India ² Department of Transfusion Medicine, Medanta- The Medicity, Gurgaon, Haryana, India ³ Hemogenomics Pvt Ltd, Bangalore, Karnataka, India ⁴ Department of Medicine, AIIMS, New Delhi, India ⁵ Department of Transfusion Medicine, AIIMS, New Delhi, India Correspondence Address: Sujatha Sunil, Vector Borne Diseases Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi - 110 067 India Source of Support: None, Conflict of Interest: None DOI: 10.4103/ajts.AJTS_108_19

OBJECTIVES: The study objective was evaluation of amotosalen and ultraviolet A (UVA) illumination-based inactivation of dengue virus (DENV) in blood platelets. MATERIALS AND METHODS: Whole blood was collected from healthy donors and platelet concentrates were prepared at a tertiary care hospital in Gurugram, India. Platelet units collected from five blood group matched individuals were pooled and spiked with DENV. The spiked platelet units were subjected to amotosalen treatment followed by UVA illumination, to evaluate the efficiency of this method for viral inactivation. The treated platelet units were evaluated for the presence of infectious DENV. Amotosalen levels were quantified in the treated samples using high-performance liquid chromatography. RESULTS: The presence of replicating DENV was not observed in spiked platelet units treated with amotosalen and UVA illumination, whereas untreated units contained actively replicating DENV. Amotosalen levels were found to be in the permissible range after photochemical inactivation. CONCLUSIONS: Amotosalen/UVA pathogen inactivation treatment showed efficient inactivation of DENV in platelet components. Therefore, it seems to be a promising method for mitigating the risk of dengue transmission through transfusion of potentially contaminated platelet components in dengue-endemic countries such as India.

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