Asian Journal of Transfusion Science
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LETTER TO THE EDITOR  
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Refrigerated centrifuge calibration for maximum platelet and plasma yield in a blood bank


 Department of Transfusion Medicine, Jawaharlal Institute of Post Graduate Medical Education and Research, Puducherry, India

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Date of Submission22-May-2019
Date of Decision06-Apr-2021
Date of Acceptance06-Jun-2021
Date of Web Publication04-Jun-2022
 


How to cite this URL:
Loganathan R, Abhishekh B. Refrigerated centrifuge calibration for maximum platelet and plasma yield in a blood bank. Asian J Transfus Sci [Epub ahead of print] [cited 2022 Jul 6]. Available from: https://www.ajts.org/preprintarticle.asp?id=345999


Sir,

Platelet yield by centrifugation separation from whole blood depends on donor platelet count, whole blood separation techniques, speed, acceleration, deceleration, and time used for platelet preparation.[1],[2] It is necessary that every center adopts its centrifuge setting for maximum platelet yield. The final platelet concentration of the random donor platelet (RDP) product has much bearing on the therapeutic efficacy in the recipient. Our center uses cryofuge 6000i for component preparation. After a minor maintenance of the equipment, we found that the initial settings which were 1400 rpm for 9 minutes with 9 acceleration and 7 deceleration were not yielding satisfactory quality parameters. We tried 6 different combinations for platelet-rich plasma (PRP) preparation, i.e., only light spin was altered and our second heavy spin was constant (3400 rpm for 10 minutes with 9 acceleration and 7 deceleration). During routine bleeding, 2 ml EDTA sample was collected along with pilot sample and complete blood count (CBC) were done by cell counter. On each setting, about 5 samples were collected. Whole blood was centrifuged at 6 different settings as shown in [Table 1]. After centrifugation, PRP was expressed from whole blood bag into platelet transfer bag and the mother bag was clamped. The PRP bag was mixed thoroughly to get a homogenous representative sample from the PRP bag under sterile closed-system conditions. The representative PRP sample was sent for CBC using cell counter.
Table 1: Average platelet yield, average plasma yield, average total platelet yield in platelet-rich plasma bag and average total platelet in random donor platelet bags by different settings

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   Calculations Top


Whole blood volume = 350 ml

PRP volume was obtained by weighing PRP bag after light spin and converting it into volume.


   Formula Top


Average yield in platelet bag, average plasma yield, average total platelet in PRP[4] bag, and average total platelet in RDP bags are assessed as depicted in [Table 1].

All the values have been detailed and enumerated in [Table 2]. By analysis, we established that setting number IV has given maximum platelet and plasma yield, average total platelet in PRP bag and in RDP bag.
Table 2: Calculation of platelet yield in platelet-rich plasma and platelet concentrate

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It is always imperative to choose the program with minimal duration and rpm which gives the most acceptable yield.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
   References Top

1.
Singh RP, Marwaha N, Malhotra P, Dash S. Quality assessment of platelet concentrates prepared by platelet rich plasma-platelet concentrate, buffy coat poor-platelet concentrate (BC-PC) and apheresis-PC methods. Asian J Transfus Sci 2009;3:86-94.  Back to cited text no. 1
[PUBMED]  [Full text]  
2.
Fung MK, Grossman BJ, Hillyer CD, Westhoff CM. Technical Manual. 17th ed. Maryland: American Association of Blood Banks; 2014.  Back to cited text no. 2
    
3.
Saran R. Transfusion Medicine Technical Manual. 2nd ed. New Delhi: Directorate General of Health Services; 2003.  Back to cited text no. 3
    
4.
Keegan T, Heaton A, Holme S, Owens M, Nelson E, Carmen R. Paired comparison of platelet concentrates prepared from platelet-rich plasma and buffy coats using a new technique with 111In and 51Cr. Transfusion 1992;32:113-20.  Back to cited text no. 4
    

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Correspondence Address:
R Loganathan,
Department of Transfusion Medicine, Jawaharlal Institute of Post Graduate Medical Education and Research, Puducherry - 605 006
India
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/ajts.ajts_56_19




 
 
    Tables

  [Table 1], [Table 2]



 

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2006 - Asian Journal of Transfusion Science | Published by Wolters Kluwer - Medknow
Online since 10th November, 2006