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Ferric reducing ability of plasma: A potential marker in stored plasma
Carl Hsieh, Soumya Ravikumar, Vani Rajashekharaiah
Department of Biotechnology, School of Sciences, Jain University, Bengaluru, Karnataka, India
Correspondence Address:
Vani Rajashekharaiah,
Department of Biotechnology, School of Sciences, Jain University, 18/3, 9th Main, 3rd Block, Jayanagar, Bengaluru - 560 011, Karnataka
India
 Source of Support: None, Conflict of Interest: None DOI: 10.4103/ajts.AJTS_96_17
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BACKGROUND: The ferric reducing ability of plasma (FRAP) assay is used for measuring the antioxidant capacity. FRAP is proportional to the molar concentration of the antioxidant capacity.
AIM: The objective of this study attempts to analyze the possibilities of FRAP as an indicator of oxidative stress (OS).
SETTINGS AND DESIGN: Blood was drawn from male Wistar rats and stored over a period of 20 days at 4°C in citrate phosphate dextrose adenine-1 (CPDA-1). They were divided into two groups: controls and experimentals. The experimentals were added with antioxidants-L-carnitine, curcumin (Cu), Vitamin C (VC), and caffeic acid (CA) of varying concentrations-10, 30, and 60 mM (n = 5 for each group).
MATERIALS AND METHODS: Plasma was isolated from these samples at regular intervals (every 5 days) and FRAP and Protein were assayed. Results were analyzed by two-way ANOVA, using GraphPad Prism 6 (GraphPad Software, Inc. USA).
RESULTS: FRAP was maintained in controls. VC (ascorbic acid) was the most potent antioxidant in terms of FRAP during storage compared to the above antioxidants.
CONCLUSION: This study emphasizes the use of FRAP as a potential marker of OS in plasma of stored blood. FRAP can be utilized as a reliable marker for determining the antioxidant capacity.
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