Asian Journal of Transfusion Science

CASE REPORT
Year
: 2020  |  Volume : 14  |  Issue : 1  |  Page : 57--59

Applying Donath–Landsteiner test for the diagnosis of paroxysmal cold hemoglobinuria


Aseem Kumar Tiwari, Geet Aggarwal, Subhasis Mitra, Dinesh Arora, Gunjan Bhardwaj, Ankita Ratan, Divya Setya 
 Department of Transfusion Medicine, Medanta-The Medicity, Gurgaon, Haryana, India

Correspondence Address:
Dr. Aseem Kumar Tiwari
Department of Transfusion Medicine, Medanta-The Medicity, Sector-38, Gurgaon - 122 001, Haryana
India

61-year old male patient was admitted to the hospital with clinical picture of hemolytic anemia with hemoglobinuria. Patient was suspected to have Infectious Mononucleosis (IM) with Auto Immune Hemolytic Anemia (AIHA). DAT was positive with anti-C3d specificity. Donath Landsteiner (DL) test was positive; confirming Paroxysmal Cold Hemoglobinuria (PCH). The final diagnosis was IM with PCH. Patient was managed conservatively and discharged after seven days. DL test specifically detects a biphasic autoantibody (IgG type) that binds to RBCs at cold temperatures, and fixes complement on the RBC membrane. However RBCs are only lysed upon warming to 37C when complement cascade proceeds to completion.


How to cite this article:
Tiwari AK, Aggarwal G, Mitra S, Arora D, Bhardwaj G, Ratan A, Setya D. Applying Donath–Landsteiner test for the diagnosis of paroxysmal cold hemoglobinuria.Asian J Transfus Sci 2020;14:57-59


How to cite this URL:
Tiwari AK, Aggarwal G, Mitra S, Arora D, Bhardwaj G, Ratan A, Setya D. Applying Donath–Landsteiner test for the diagnosis of paroxysmal cold hemoglobinuria. Asian J Transfus Sci [serial online] 2020 [cited 2023 Feb 2 ];14:57-59
Available from: https://www.ajts.org/article.asp?issn=0973-6247;year=2020;volume=14;issue=1;spage=57;epage=59;aulast=Tiwari;type=0