Asian Journal of Transfusion Science

ORIGINAL ARTICLE
Year
: 2022  |  Volume : 16  |  Issue : 1  |  Page : 89--94

Reappraisal of screening strategy in hepatitis B surface antigen-negative blood donors: Correlation with hepatitis B virus-DNA quantification


Md Sohrab Alam1, Tushar Samdani2, Md Sawkat Hassan2 
1 Department of Immunology, Molecular Genetics Laboratory, BIRDEM General Hospital, Shahbag, Dhaka, Bangladesh
2 Department of Laboratory Medicine, Asgar Ali Hospital, Gandaria, Dhaka, Bangladesh

Correspondence Address:
Md Sohrab Alam
Department of Immunology, Molecular Genetics Laboratory, BIRDEM General Hospital, Shahbag, Dhaka
Bangladesh

BACKGROUND: In spite of screening for hepatitis B surface antigen (HBsAg), transfusion-associated hepatitis B virus (TAHBV) infection remains a serious public health problem due to transmission of HBV in window period and occult HBV infection. To avoid TAHBV infection, some health-care facilities have started Hepatitis B core antibody test along with HBsAg, but this leads to a lot of potential donor rejection who are not HBV infected. Our aim is to find a new protocol of donor screening to prevent TAHBV without compromising blood availability. MATERIALS AND METHODS: A total of 88 HBsAg-negative anti-HBc total positive blood donors were included in this study. All samples were also tested for anti-HBs by enzyme immunoassay and for the presence of HBV-DNA viral load by real-time polymerase chain reaction. RESULTS: A total of 88 HBsAg negative and anti-HBc, total positive blood donors were tested for anti-HBs and HBV-DNA (Qn.). Among them, 76 donors (86.4%) (males 73 and females 3) were found to be positive for anti-HBs, while rest 12 (13.6%) showed no detectable antibody against HBsAg. HBV-DNA was found to be positive in 4 (7.7%) donor samples among 52 (60%) who have anti-HBs level <100 mIU/ml, while 36 (40%) donor samples were found to have >100 mIU/ml anti-HBs antibody with no detectable HBV-DNA. CONCLUSION: HBV-DNA should be implemented as a screening test of the blood donors to prevent TAHBV infection without potential donor rejection.


How to cite this article:
Alam MS, Samdani T, Hassan MS. Reappraisal of screening strategy in hepatitis B surface antigen-negative blood donors: Correlation with hepatitis B virus-DNA quantification.Asian J Transfus Sci 2022;16:89-94


How to cite this URL:
Alam MS, Samdani T, Hassan MS. Reappraisal of screening strategy in hepatitis B surface antigen-negative blood donors: Correlation with hepatitis B virus-DNA quantification. Asian J Transfus Sci [serial online] 2022 [cited 2022 Oct 2 ];16:89-94
Available from: https://www.ajts.org/article.asp?issn=0973-6247;year=2022;volume=16;issue=1;spage=89;epage=94;aulast=Alam;type=0